software program turbosequest v.27 (Thermo Fisher)
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software program turbosequest v.27
Software Program Turbosequest V.27, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/turbosequest+program/pmc11059206-1065-23-26
Average 90 stars, based on 1 article reviews
Software Program Turbosequest V.27, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/turbosequest+program/pmc11059206-1065-23-26
Average 90 stars, based on 1 article reviews
software program turbosequest v.27 - by Bioz Stars,
2026-09
90/100 stars
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Tandem Mass Spectroscopy:Article Title: High-throughput analysis of rat liver plasma membrane proteome by a nonelectrophoretic in-gel tryptic digestion coupled with mass spectrometry identification. Article Snippet: In-gel digestion is commonly used after proteins are resolved by polyacrylamide gel electrophoresis (SDS-PAGE, 2-DE).. It can also be used on its own in conjunction with tandem mass spectrometry (MS/ MS) for the direct analysis of complex proteins.. Here, we describe a strategy combining isolation of purified plasma membrane, efficient digestion of plasma membrane proteins in polyacrylamide gel, and high-sensitivity analysis by advanced mass spectrometry to create a new rapid and high-throughput method. Article Title: Efficacy of a formalin-killed cell vaccine against infectious spleen and kidney necrosis virus (ISKNV) and immunoproteomic analysis of its major immunogenic proteins. Article Snippet: Infectious spleen and kidney necrosis virus (ISKNV), the type species of genus Megalocytivirus in the family Iridoviridae, is the most important etiological agents in mandarin fish industry in China.. Since its first occurrence in China in the early 1990s, there is no effective method to prevent and control this virus.. Here we report the successful development of a formalin-killed cell-cultured (FKC) vaccine again ISKNV. Article Title: Hydroxypropyl-β-cyclodextrin-mediated alterations in cell permeability, lipid and protein profiles of steroid-transforming Arthrobacter simplex. Article Snippet: Hydroxypropyl-β-cyclodextrin (HP-β-CD) enhances steroid 1-dehydrogenation biotransformation by Arthrobacter simplex.. In this work, HP-β-CD-induced improvement of A. simplex CPCC 140451 cell envelope permeability which had positive effects on the steroid bioconversion was confirmed by a comparative investigation which showed a lower dehydrogenase activity and higher cell permeability of the cells after being incubated with HP-β-CD.. Atomic force microscopy and transmission electron microscopy micrographs showed that HP-β-CD altered the size, sharpness, and surface structure of the cell envelope. Article Title: Effects of two kinds of imidazolium-based ionic liquids on the characteristics of steroid-transformation Arthrobacter simplex Article Snippet: System control and data collection were performed using Xcalibur software version 1.4 (Thermo). .. The acquired MS/MS spectra were compared against the Arthrobacter database from NCBI by using Article Title: A fully automated 2-D LC-MS method utilizing online continuous pH and RP gradients for global proteome analysis. Article Snippet: The conventional 2-D LC-MS/MS setup for global proteome analysis was based on online and offline salt gradients (step and continuous) using strong-cation-exchange chromatography in conjunction with RP chromatography and MS.. The use of the online system with step salt elution had the possibility of resulting in peptide overlapping across fractions.. The offline mode had the option to operate with continuous salt gradient to decrease peak overlap, but exhibited decreased robustness, lower reproducibility, and sample loss during the process. Article Title: Secretome-derived isotope tags (SDIT) reveal adipocyte-derived apolipoprotein C-I as a predictive marker for cardiovascular disease. Article Snippet: We developed a quantitative strategy, named secretome-derived isotopic tag (SDIT), to concurrently identify and quantify the adipocyte-secreted plasma proteins from normal and high-fat-diet (HFD) induced obese mice, based on the application of isotope-labeled secreted proteins from cultured mouse adipocytes as internal standards.. We detected 197 proteins with significant changes between normal and obese mice plasma.. Importantly, a novel adipocyte-secreted plasma protein, apolipoprotein C-I (apoC-I), significantly increased in the obese mice plasma. Sequencing:Article Title: High-throughput analysis of rat liver plasma membrane proteome by a nonelectrophoretic in-gel tryptic digestion coupled with mass spectrometry identification. Article Snippet: In-gel digestion is commonly used after proteins are resolved by polyacrylamide gel electrophoresis (SDS-PAGE, 2-DE).. It can also be used on its own in conjunction with tandem mass spectrometry (MS/ MS) for the direct analysis of complex proteins.. Here, we describe a strategy combining isolation of purified plasma membrane, efficient digestion of plasma membrane proteins in polyacrylamide gel, and high-sensitivity analysis by advanced mass spectrometry to create a new rapid and high-throughput method. Article Title: Secretome-derived isotope tags (SDIT) reveal adipocyte-derived apolipoprotein C-I as a predictive marker for cardiovascular disease. Article Snippet: We developed a quantitative strategy, named secretome-derived isotopic tag (SDIT), to concurrently identify and quantify the adipocyte-secreted plasma proteins from normal and high-fat-diet (HFD) induced obese mice, based on the application of isotope-labeled secreted proteins from cultured mouse adipocytes as internal standards.. We detected 197 proteins with significant changes between normal and obese mice plasma.. Importantly, a novel adipocyte-secreted plasma protein, apolipoprotein C-I (apoC-I), significantly increased in the obese mice plasma. Software:Article Title: High-throughput analysis of rat liver plasma membrane proteome by a nonelectrophoretic in-gel tryptic digestion coupled with mass spectrometry identification. Article Snippet: In-gel digestion is commonly used after proteins are resolved by polyacrylamide gel electrophoresis (SDS-PAGE, 2-DE).. It can also be used on its own in conjunction with tandem mass spectrometry (MS/ MS) for the direct analysis of complex proteins.. Here, we describe a strategy combining isolation of purified plasma membrane, efficient digestion of plasma membrane proteins in polyacrylamide gel, and high-sensitivity analysis by advanced mass spectrometry to create a new rapid and high-throughput method. Article Title: Efficacy of a formalin-killed cell vaccine against infectious spleen and kidney necrosis virus (ISKNV) and immunoproteomic analysis of its major immunogenic proteins. Article Snippet: Infectious spleen and kidney necrosis virus (ISKNV), the type species of genus Megalocytivirus in the family Iridoviridae, is the most important etiological agents in mandarin fish industry in China.. Since its first occurrence in China in the early 1990s, there is no effective method to prevent and control this virus.. Here we report the successful development of a formalin-killed cell-cultured (FKC) vaccine again ISKNV. Article Title: Hydroxypropyl-β-cyclodextrin-mediated alterations in cell permeability, lipid and protein profiles of steroid-transforming Arthrobacter simplex. Article Snippet: Hydroxypropyl-β-cyclodextrin (HP-β-CD) enhances steroid 1-dehydrogenation biotransformation by Arthrobacter simplex.. In this work, HP-β-CD-induced improvement of A. simplex CPCC 140451 cell envelope permeability which had positive effects on the steroid bioconversion was confirmed by a comparative investigation which showed a lower dehydrogenase activity and higher cell permeability of the cells after being incubated with HP-β-CD.. Atomic force microscopy and transmission electron microscopy micrographs showed that HP-β-CD altered the size, sharpness, and surface structure of the cell envelope. Article Title: Effects of two kinds of imidazolium-based ionic liquids on the characteristics of steroid-transformation Arthrobacter simplex Article Snippet: System control and data collection were performed using Xcalibur software version 1.4 (Thermo). .. The acquired MS/MS spectra were compared against the Arthrobacter database from NCBI by using Article Title: A fully automated 2-D LC-MS method utilizing online continuous pH and RP gradients for global proteome analysis. Article Snippet: The conventional 2-D LC-MS/MS setup for global proteome analysis was based on online and offline salt gradients (step and continuous) using strong-cation-exchange chromatography in conjunction with RP chromatography and MS.. The use of the online system with step salt elution had the possibility of resulting in peptide overlapping across fractions.. The offline mode had the option to operate with continuous salt gradient to decrease peak overlap, but exhibited decreased robustness, lower reproducibility, and sample loss during the process. Article Title: Secretome-derived isotope tags (SDIT) reveal adipocyte-derived apolipoprotein C-I as a predictive marker for cardiovascular disease. Article Snippet: We developed a quantitative strategy, named secretome-derived isotopic tag (SDIT), to concurrently identify and quantify the adipocyte-secreted plasma proteins from normal and high-fat-diet (HFD) induced obese mice, based on the application of isotope-labeled secreted proteins from cultured mouse adipocytes as internal standards.. We detected 197 proteins with significant changes between normal and obese mice plasma.. Importantly, a novel adipocyte-secreted plasma protein, apolipoprotein C-I (apoC-I), significantly increased in the obese mice plasma. Virus:Article Title: Efficacy of a formalin-killed cell vaccine against infectious spleen and kidney necrosis virus (ISKNV) and immunoproteomic analysis of its major immunogenic proteins. Article Snippet: Infectious spleen and kidney necrosis virus (ISKNV), the type species of genus Megalocytivirus in the family Iridoviridae, is the most important etiological agents in mandarin fish industry in China.. Since its first occurrence in China in the early 1990s, there is no effective method to prevent and control this virus.. Here we report the successful development of a formalin-killed cell-cultured (FKC) vaccine again ISKNV. |